banana dna extraction lab report conclusion

Experiment to purify DNA from fruit Bananas, kiwis and strawberries all work well. Equipment You will need the following: Two clear glasses/cups Sealable plastic bag Banana Blunt knife and teaspoon Plate/chopping board Measuring jug << The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. the DN +co$or' texture', The soap so$ut#o" co"ta#"s so%#um $aure$ su$!ate' wh#ch, the soap pu$$s apart the !ats +$#p#%s a"% prote#"s that. It is also essential for carrying out forensic science, sequencing genomes, detecting bacteria and viruses in the environment and for determining paternity. Mashing the banana exposes a greater surface area from which to extract the DNA. the so$ut#o" whe" the a$coho$, #s a%%e%. 6T3G]Zq`S;z*H27$21a4a@ aaSn;,8@ U By continuing well assume youre on board with our o/ H|9 O7ze~.p -s'NK SNlg7WO dq=}i43 DNA contains the instructions required to build each living thing. Step 8: Pour the drained liquid into a glass. Overall, the Lab: Extracting DNA from Bananas demonstrated and visualized the process of how DNA can be seen from the naked eye by removing DNA directly from bananas. essay, Genome Patterns of Common DNA Variations in Three Human Populations, Strawberry DNA Extraction Lab Formal Write Up, Next Generation DNA Sequencing Technologies Biology Essay, Write What is the best fruit to extract DNA from? Genetic Engineering of Plants. //= $post_title Each living organism has its own unique DNA sequence. Do not lower the bag or mashed the banana too close to the ZIP seal on the bag. Course Hero is not sponsored or endorsed by any college or university. REFERENCES: BOOKS: * Neil A. Campbell, Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson, Biology (8th Ed), Pearson International Edition: Pearson, Benjamin Cummings. ASU - Ask A Biologist. The Essay on Human Genome Project Sequence Dna Information. Result Photo 1: Test tubes containing solution S and SS CONCLUSION. About 60 percent of our genes have a recognizable counterpart in the banana genome! The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. DNA extraction The concentrations of the extracted DNA was measured using the NanoPhotometer,UV/Vis spectrophotometer (Implen, munich, Germany) and the average concentration for 12 samples was 0.045g/l. << /ca 1.0 0000003225 00000 n 4.1. 1 1 . Banana DNA Experiment This DNA extraction is simple and works every time but the DNA is not pure. ASU - Ask A Biologist. Therapeutic Lifestyle Changes for a Happier, Healthier and Longer Life. >> /4 When looking at DNA extraction,the key steps for isolating DNA from a plant cell or animal cell is to the solution is minimal remove the banana residue and transfer 5mL of the filtrate into a separate dry gas tube. After stirring for five to ten minutes, the mixture of banana and shampoo was filtered by pouring it into the coffee filter and letting the solution drain for several minutes until there was approximately five milliliters of filtrate to test. 6. Some of the protein is still present. DNA extraction is integral to the process of genetic modification of plants. Some of the protein is still present. Body identification is a subfield of forensic science wherein investigators need to identify a body. DNA can be extracted and observed. For more info, see, https://askabiologist.asu.edu/activities/banana-dna, Public Service and * To properly and successfully extract DNA from banana using cell disruption and separation techniques. Updated on April 13, 2019 Extracting DNA from a banana may sound like a difficult task, but it is not very difficult at all. Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . 19 April, 2016. https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. 135 32 These cookies track visitors across websites and collect information to provide customized ads. /Width 625 can use them for free to gain inspiration and new creative ideas for their writing 2 Mar 2023. https://askabiologist.asu.edu/activities/banana-dna. Complementarity serves as the basis for both the replication of DNA and for the transcription of DNA into RNA. 4 0 obj 1. 0000002514 00000 n After the alcohol layer is set up, wait for eight minutes. The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs. 0000045111 00000 n %PDF-1.4 % It is incredibly small, but we can see it by extracting DNA and isolating chains of it. DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. Need urgent help with your paper? 0000002685 00000 n Segments, or pieces, of DNA are called "genes." Hear Let this two-layer mixture sit for eight minutes. Some additional protocols for DNA extractions: Sex Influenced Female Dominant Inheritance, beakers 100ml and 2x250ml (or jars that same size), liquid dishwashing detergent (clear solution works better than cloudy), Make a 10% solution of dishwashing detergent in one of your 250ml beakers (make enough to fill the mortar half way), Add the 10% detergent solution to cover the fruit, Grind the banana in the soap solution until there are no lumps (this is the hardest part, have the students take turns), Fasten a coffee filter to the 250 ml beaker with a rubber band so there is room for the fruit to be poured into the filter, Pour the fruit and soap mixture into the filter and place in a cold place to filter, Clean up your lab area while you are waiting for the filtering to complete, Remove the filter and throw away the contents, Pour enough of the liquid into a test tube to fill it 2 cm high, Add twice the volume of 95% ethanol (try to layer the alcohol on top by gently pouring it down the side of the test tube), The DNA will precipitate in the boundary between the alcohol and the banana mixture as a cloudy mass. Within every living organism, most cells contain a complete set of DNA instructions. 20 milliliters (four teaspoons) of distilled water was then added to the mixture. In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent soap then continue macerating.Once a thick pace called We are going to use fruits because they are soft and easy to pulverize. <> (c) Or, slowly pour the alcohol down the inside of the test tube with a pasteur pipette or medicine dropper. Because DNA is in every cell, there is a lot of it in an organism. 0000001999 00000 n It holds the instructions necessary for the organism to grow and function, and is passed on from generation to generation through heredity. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. It also controls many of the features that make an organism unique. stream We even have genes in common with the banana plant! 7. A very simple and inexpensive method for high-throughput DNA extraction from animal tissues has been developed. When you are done, take a closer look at the stuff on the stirrer. DNA molecule exists in cells as a long coiled structure often described as a double helix. Throughout the entire experiment there were many possible ways that the ending result of the appearance of the DNA could have differed. 6 (5 points) Which of the applications of genomics listed below can be used in agriculture? The Mendelian Revolution: The Emergence of Hereditarian Concepts in Modern Science and Society. Try to avoid making too much foam. Banana DNA Extraction Lab Name: _____ Objectives: Describe where DNA is located in a plant cell. 3 0 obj When we added. /CreationDate (D:20220315063300+02'00') These comprise the genetic alphabet which in various combinations, will specify the components of proteins. This could be a problem if two bacteria looked similar because they could easily. This DNA extraction is simple and works every time but the DNA is not pure. >> Why is working with linked genes not simple? Dna Banana Extraction Lab Answers Recognizing the pretentiousness ways to get this books dna banana extraction lab answers is additionally useful. 3g PROCEDURE (A) Extraction solution recipe: 4ml of shampoo was mixed with 36ml of distilled water. Lastly, when good results were obtained, there was enough DNA to spool onto a rod or a plastic loop. DNA is organized into genes and stores genetic information. 20g of banana was added into each ziplock bag labeled 'Extraction 1' and 'Extraction 2' 3. Publisher: Arizona State University School of Life Sciences Ask A Biologist. But with bananas, we share about 50 percent of our genes, which turns out to be only about 1 percent of our DNA, emails Mike Francis, a Ph. /BitsPerComponent 8 The solution was stirred well and slowly. Then a plastic pipette was filled with the filtrate two times and added to the alcohol. <<37B5D6E5CA566B46AF6D8E49138E4E7A>]>> /Type /Catalog endobj 19 Apr 2016. 0000003969 00000 n /Type /ExtGState The white DNA could be seen precipitating out of the alcohol layer. As a human, you usually get a set of instructions from your mom and another set from your dad more, Molecule: a chemical structure that has two or more atoms held together by a chemical bond. The nanodrop system revealed that at an absorption of 260 wavelengths, the DNA measured 7.49 using a 374.66 ng/ul sample. These instructions are in segments of DNA called genes. The filtrate, or filtered solution from the bag, was then placed into a test tube. Furthermore, analysis of forensic evidence is defined as the application of forensic science and technology to identify specific objects from the trace evidence they leave, often at a crime scene or the scene of an accident. /Title () Then eight millilitres of DNA buffer was then added to the pure. 1 ) Peel a banana and take about of it 2 ) In a ziplock bag , mash the banana until it is a liquid 3 ) Mix 20 m L of the extraction buffer with the banana liquid The goal of this lab is to extract the genetic material from an organism so that it can be seen with the naked eye . DNA or deoxyribonucleic acid is found in all living things. /Height 155 Look over some of the links provided and develop a hypothesis and design an experiment that will allow you to perform your own DNA extraction. A water bath was prepared. 1 2 . However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. Get expert help in mere %PDF-1.4 % Then the bananas were mushed more. re$ease% !rom the ce$$. Video of this experiment can be found here: DN #s !ou"% #" the ce$$s o! These engineerings have created ballyhoo among scientists since they enable inexpensive and faster. Then alcohol added to the solution causes the DNA to precipitate out. The test tube solution. At an absorption of 280 wavelengths, the DNA measured 3.802 using a 374.66 ng/ul sample. DNA Extraction Lab Begin by watching the Video of a Strawberry DNA extraction, also available in the module, and think about how DNA extraction is possible. endobj Conclusion Purpose : This lab was conducted in order to show and analyze the way DNA is extracted. What is the purpose of extracting DNA from banana? Isopropyl rubbing alcohol allows me to see the DNA because when molecules are insoluble the clump together and become visible so the alcohol makes the DNA insoluble making it become visible. We understood how a buffer solutions disrupts the plasma membrane and releasing cellular components into the solution. For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. 0 As part of the chromosomes, the information contained in genes can be transmitted faithfully by parents through gametes to their offspring. In a separate five-ounce cup, a solution consisting of one teaspoon of shampoo and two pinches of salt was made. your own essay or use it as a source, but you need Step 1: Chop up the banana. There are four types of nucleotides which vary depending on the four nitrogenous bases of the molecule. 135 0 obj <> endobj ?>. Studying DNA is one way scientists learn about what is necessary for life. This cookie is set by GDPR Cookie Consent plugin. During a DNA extraction, a detergent will cause the cell to pop open, or lyse, so that the DNA is released into solution.

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